You can try the following modification of your code:
audio = request.files['audio_file']
The request.files is a dictionary. The dictionary key that will allow you to retrieve the audio file is 'audio_file' instead of 'audio'.
request.files
you can use save() function
audio = request.files["audio"] path='./videos/sample.wav'; audio.save(path)
check for further detailshttps://werkzeug.palletsprojects.com/en/2.0.x/datastructures/#werkzeug.datastructures.FileStorage.save