Tkinter removing a button from a running program

self.call_button is set to the result of grid(row=5, column=5) and not to the Button..

from tkinter import *class App(Frame):    def __init__(self, master):        Frame.__init__(self, master)        self.grid()        self.a()    def a(self):        self.call_button = Button(self, text = "Call", command=self.b)        self.call_button.grid(row=5, column=5) # This is fixing your issue    def b(self):        self.call_button.destroy()root = Tk()app = App(master=root)app.mainloop()

In python, if you do foo=a().b(), foo is given the value of b(). So, when you do self.call_button = Button(...).grid(...), self.call_button is given the value of .grid(...), which is always None.

if you want to keep a reference to a widget, you need to separate your widget creation from your widget layout. This is a good habit to get into, since those are conceptually two different things anyway. In my experience, layout can change a lot during development, but the widgets I use don't change that much. Separating them makes development easier. Plus, it opens the door for later if you decide to offer multiple layouts (eg: navigation on the left, navigation on the right, etc).